Proven Bioavailability and absorption with High Molecular Weight HA

Oral Absorption Study

Washington. D.C., April 18, 2004 - A consortium of scientists released clinical research results about oral delivery of radiolabeled Hyaluronan (HA) and its ability to be taken up by joints. Dr. Alex Schauss, Director of AIBMR, presented the findings at the 2004 Experimental Biology conference, conducted by the Federation of American Societies for Experimental Biology.

Hyaluronan has been used for years in veterinary and human medicine as an injection to replace lost joint fluid. The study was led by Dr. Schauss and conducted by a consortium of scientists from the Life Sciences Division of the American Institute for Biosocial and Medical Research Inc. in Puyallup, Washington and the National "FJC" Research Institute for Radiobiology and Radiohygiene, National Institute for Health in Budapest, Hungary. The research was supported by Weider Nutrition International.

"This is the first time hyaluronic acid has been reported to be absorbed orally, which paves the way for HA dietary supplements to be introduced and deliver on anti-aging and joint health promises," said Dr. Luke Bucci, Ph.D., Vice President of Research for Weider Nutrition International.

Until now, there was no data on pharmacokinetics after oral intake and the therapeutic use of hyaluronic acid was limited to injections or topical applications. The results of this study, which examined the absorption, excretion and distribution of radiolabeled HA after a single oral administration in Wistar rats and Beagle dogs, demonstrated that HA is absorbed and distributed to organs and joints after a single oral administration.

Excerpt from Abstract # 129.4- Absorption, distribution and excretion of 99mtechnetium labeled hyaluronan after single oral doses in rats and beagle dogs.

This study examined the absorption, excretion and distribution of radiolabeled hyaluronan (HA) after a single oral administration in Wistar rats and Beagle dogs. High Molecular Weight HA from bacterial fermentation was radioactively labelled with 99-technetium and its oral uptake in rats and dogs was studied. Uptake of HA into the bloodstream (5% of an oral dose) was found, and scintigraphy found uptake of labelled HA into joints (FASEB, 2004). A. G. Schauss, L. G. Balogh, A. G. Polyak, D. G. Mathe, R. G. Kiraly and G. G. Janoki American Institute for Biosocial and Medical Research, Inc and National Institute for Health.

Thus, purified, high molecular weight HA is bioavailable and delivered to joints. From these results, approximately 4.5 billion molecules of HA may reach each knee joint after oral ingestion of 3 mg of HA in humans. These results demonstrate that HA is absorbed and distributed to organs and joints after a single oral administration.

Molecular Weight of HA

The molecular weight is important!

Or........Size Matters!

HA is 2.1 - 2.5 million Daltons.

The molecular weight of HA in the Lymphatic and healthy joint fluid is 1-2 million Daltons*, and equivalent to 3.5mg per ml of fluid, therefore the HA is a compatible size for the lymph fluid where it is transported.

*A Dalton is a measure of the mass of an atom that whirls around the nucleus. Atoms are the smallest particles of matter. The nucleus is the cell's control centre.